Measurement of progesterone in sheep using a commercial ELISA kit for human plasma

Determination of serum or plasma progesterone (P4) concentrations is important to recognize pregnant and non-pregnant ewes, and also to predict the number of carried lambs. The 2 most common methodologies for the detection of plasma P4 are radioimmunoassay (RIA) and enzyme immunoassay (EIA). RIA is very expensive, and not all laboratories are equipped to perform this test; EIA is commercially available for human use, but only a few companies produce species-specific kits, which are expensive. We verified for ovine plasma a less expensive and easily available ELISA kit (DiaMetra) designed to quantify P4 in humans.
Pools of ovine and human plasma were used to compare repeatability, accuracy, sensitivity, and stability of P4 measured by the DiaMetra kit. Repeatability data were within 15%, and accuracy values were ~90% for both plasma matrices. Stability data showed a loss of <20% for freeze-thaw and <30% for 30-d storage. All parameters were acceptable under international guidelines for method validation. The human ELISA kit was used successfully to quantify plasma P4 in 26 ewes during pregnancy until delivery. P4 concentrations were also correlated with the number of carried lambs.

Comparison of the Utility of Recombinant B8/2 Subunit of the Antigen B, Native Antigen, and a Commercial ELISA Kit in the Diagnosis of Human Cystic Echinococcosis

Cystic echinococcosis (CE) is a helminthic disease caused by the larval form of Echinococcus granulosus. In the present study, the B8/2 subunit of antigen B (AgB) of E. granulosus was expressed in E. coli host and then applied in a diagnostic ELISA set up.
The DNA sequence of AgB8/2 subunit from E. granulosus was extracted from the GenBank and codon-optimized. The target sequence was cloned in an expression vector (pGEX-4T-1). The produced antigen was used in an ELISA system, and its performance for the diagnosis of human hydatid cyst was evaluated, using sera from CE and non-CE patients, along with the sera from healthy subjects.
Moreover, the diagnostic value of the recombinant protein was compared with native AgB, as well as with a commercial kit.
Antibodies to hydatid cyst were detected in 27 out of 30 patients corresponding to a sensitivity of 90% (95% CI: 73-98%). Cross-reaction with sera of non-CE subjects was seen in two cases resulted in a specificity of 93.5% (95% CI: 82-98%) for the test. A sensitivity of 87% and specificity of 90% were found for the native form of the antigen, while the ELISA commercial kit had a sensitivity of 97% and specificity of 95%.
Our data show that rEgAgB8/2 is an appropriate source of antigen for the serological diagnosis of human hydatid cyst. Co-expression of the rEgAgB/2 along with other subunits of AgB may enhance the performances of these antigens for the serodiagnosis of human CE.

Unreliability of three commercial Coxiella burnetii phase II IgM ELISA kits for the seroscreening of acute Q fever in human cases

Seroprevalence of Q fever (QF) caused by Coxiella burnetii has been reported from different parts of India. Usually serological/molecular tests are employed for detection of infection. The present study was undertaken to verify the validity of three different QF phase II IgM ELISA kits for acute QF diagnosis by comparing with the gold standard indirect fluorescent antibody assay (IFA).
Fifty eight serum samples collected from 42 patients (26 patients provided acute sample only and 16 both acute and convalescent samples) which were examined by all three commercial kits, were cross-checked with QF Phase II IgM IFA for confirmation.
Eleven patients were positive for C. burnetii antibodies by IFA in acute and/or convalescent serum samples. Taking IFA as a reference, percentages of sensitivity, specificity, positive predictive value and negative predictive value for Virion-Serion/Vircell/NovaTec were 36.36, 61.29, 25.00, 73.08; 81.82, 35.48, 31.03, 84.62 and 100, 25.81, 32.35, 100 per cent, respectively.
The three different ELISA kits exhibited poor agreement amongst them and unacceptable level of false positivity. IFA remains to be the only option for diagnosing acute QF. Discrepancy between the clinical findings and IFA/ELISA results needs confirmation by C. burnetii DNA detection in real-time polymerase chain reaction.
Lactate Human Elisa Kit

Lactate Human Elisa Kit

Clinical application of homemade ELISA kit for anti-human phospholipase A2 receptor antibody

To develop and evaluate an ELISA kit for Anti-PLA2R IgG. Recombinant M-type phospholipase A2 receptor (PLA2R) protein expressed in HEK293 was taken as coating antigen, HRP-labeled rabbit anti-human IgG was taken as a tracer, to test the anti-PLA2R IgG on the basis of the principle of ELISA. The detection linear range, accuracy, linear correlation, repeatability, and stability were evaluated. In addition, we made a comparison with enrolled anti-PLA2R IgG kit.
The detection linear range of the kit is no more than 2 RU/mL. The relative deviation of the kit accuracy is in -15%-15%. There is a linear correlation coefficient of higher than 0.990 0 within 2-500 RU/mL range. The CV of the repeatable test is lower than 15% when the kit was put in 37 ℃ one week, 2-8 ℃ one year, the performance remains. The consistency of testing with comparison in enrolled anti-PLA2R IgG kit is 98.9% (positive: 97.2%, negative: 100%). The Anti-PLA2R IgG ELISA Kit which we developed is nearly identical to the reference standard in specific and sensitive of clinics. It’s successfully used to determine anti-PLA2R titer and help clinical diagnosis.

Comparison of Thymic Stromal Lymphopoietin Concentration in Various Human Biospecimens from Asthma and COPD Patients Measured with Two Different ELISA Kits.

Thymic stromal lymphopoietin (TSLP) seems a promising asthma biomarker. In earlier studies, mainly the serum concentration of TSLP was investigated. The aim of the present study was to compare the TSLP concentration measured by two different ELISA kits in the serum, induced sputum, and exhaled breath condensate in asthma, COPD, and control subjects.

Lactate ELISA Kit| General Lactate ELISA Kit

EF019605 Lifescience Market 96 Tests 689 EUR

Human Lactate ELISA Kit

QY-E05474 Qayee Biotechnology 96T 361 EUR

Lactate (human) ELISA Kit

E4341-100 Biovision 805 EUR

Custom Antibody titration by ELISA up to 2 rabbits and 1 bleed

ELISA-1 Alpha Diagnostics 1 202 EUR

D-Lactate ELISA Kit| General D-Lactate ELISA Kit

EF019607 Lifescience Market 96 Tests 689 EUR

Human Lactate Dehydrogenase ELISA kit

E01L0006-192T BlueGene 192 tests 1270 EUR

Human Lactate Dehydrogenase ELISA kit

E01L0006-48 BlueGene 1 plate of 48 wells 520 EUR

Human Lactate Dehydrogenase ELISA kit

E01L0006-96 BlueGene 1 plate of 96 wells 685 EUR

Human Lactate Dehydrogenase (LDH) ELISA Kit

SEB864Hu-10x96wellstestplate Cloud-Clone 10x96-wells test plate 4502.43 EUR

Human Lactate Dehydrogenase (LDH) ELISA Kit

SEB864Hu-1x48wellstestplate Cloud-Clone 1x48-wells test plate 458.44 EUR

Human Lactate Dehydrogenase (LDH) ELISA Kit

SEB864Hu-1x96wellstestplate Cloud-Clone 1x96-wells test plate 612.05 EUR

Human Lactate Dehydrogenase (LDH) ELISA Kit

SEB864Hu-5x96wellstestplate Cloud-Clone 5x96-wells test plate 2454.23 EUR

Human Lactate Dehydrogenase (LDH) ELISA Kit

4-SEB864Hu Cloud-Clone
  • 4553.00 EUR
  • 2405.00 EUR
  • 613.00 EUR
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells

Human Lactate Dehydrogenase(LDH)ELISA Kit

GA-E0764HM-48T GenAsia Biotech 48T 289 EUR

Human Lactate Dehydrogenase(LDH)ELISA Kit

GA-E0764HM-96T GenAsia Biotech 96T 466 EUR

Human Lactate Dehydrogenase,LDH ELISA Kit

201-12-0748 SunredBio 96 tests 440 EUR

Human Lactate Dehydrogenase (LDH) ELISA Kit

20-abx152149 Abbexa
  • 7112.00 EUR
  • 3792.00 EUR
  • 879.00 EUR
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Lactate Dehydrogenase ELISA Kit (LDH)

RK01767 Abclonal 96 Tests 521 EUR

Human Lactate Dehydrogenase(LDH)ELISA Kit

QY-E01733 Qayee Biotechnology 96T 394 EUR

Human Lactate Dehydrogenase,LDH ELISA Kit

CN-03509H1 ChemNorm 96T 442 EUR

Human Lactate Dehydrogenase,LDH ELISA Kit

CN-03509H2 ChemNorm 48T 293 EUR

Human L- Lactate Dehydrogenase ELISA Kit

ELA-E1370h Lifescience Market 96 Tests 824 EUR

Human D Lactate Dehydrogenase ELISA kit

E01D0243-192T BlueGene 192 tests 1270 EUR

Human D Lactate Dehydrogenase ELISA kit

E01D0243-48 BlueGene 1 plate of 48 wells 520 EUR

Human D Lactate Dehydrogenase ELISA kit

E01D0243-96 BlueGene 1 plate of 96 wells 685 EUR

Human Lactate Dehydrogenase L ELISA kit

E01L0240-192T BlueGene 192 tests 1270 EUR

Human Lactate Dehydrogenase L ELISA kit

E01L0240-48 BlueGene 1 plate of 48 wells 520 EUR

Human Lactate Dehydrogenase L ELISA kit

E01L0240-96 BlueGene 1 plate of 96 wells 685 EUR

Monkey Lactate Dehydrogenase ELISA kit

E09L0006-192T BlueGene 192 tests 1270 EUR

Monkey Lactate Dehydrogenase ELISA kit

E09L0006-48 BlueGene 1 plate of 48 wells 520 EUR

Monkey Lactate Dehydrogenase ELISA kit

E09L0006-96 BlueGene 1 plate of 96 wells 685 EUR

Dog Lactate Dehydrogenase ELISA kit

E08L0006-192T BlueGene 192 tests 1270 EUR

Dog Lactate Dehydrogenase ELISA kit

E08L0006-48 BlueGene 1 plate of 48 wells 520 EUR

Dog Lactate Dehydrogenase ELISA kit

E08L0006-96 BlueGene 1 plate of 96 wells 685 EUR

Mouse Lactate Dehydrogenase ELISA kit

E03L0006-192T BlueGene 192 tests 1270 EUR

Mouse Lactate Dehydrogenase ELISA kit

E03L0006-48 BlueGene 1 plate of 48 wells 520 EUR

Mouse Lactate Dehydrogenase ELISA kit

E03L0006-96 BlueGene 1 plate of 96 wells 685 EUR

Rat Lactate Dehydrogenase ELISA kit

E02L0006-192T BlueGene 192 tests 1270 EUR

Rat Lactate Dehydrogenase ELISA kit

E02L0006-48 BlueGene 1 plate of 48 wells 520 EUR

Rat Lactate Dehydrogenase ELISA kit

E02L0006-96 BlueGene 1 plate of 96 wells 685 EUR

Pig Lactate Dehydrogenase ELISA kit

E07L0006-192T BlueGene 192 tests 1270 EUR

Pig Lactate Dehydrogenase ELISA kit

E07L0006-48 BlueGene 1 plate of 48 wells 520 EUR

Pig Lactate Dehydrogenase ELISA kit

E07L0006-96 BlueGene 1 plate of 96 wells 685 EUR

Goat Lactate Dehydrogenase ELISA kit

E06L0006-192T BlueGene 192 tests 1270 EUR

Goat Lactate Dehydrogenase ELISA kit

E06L0006-48 BlueGene 1 plate of 48 wells 520 EUR

Goat Lactate Dehydrogenase ELISA kit

E06L0006-96 BlueGene 1 plate of 96 wells 685 EUR

Rabbit Lactate Dehydrogenase ELISA kit

E04L0006-192T BlueGene 192 tests 1270 EUR

Rabbit Lactate Dehydrogenase ELISA kit

E04L0006-48 BlueGene 1 plate of 48 wells 520 EUR

Rabbit Lactate Dehydrogenase ELISA kit

E04L0006-96 BlueGene 1 plate of 96 wells 685 EUR

Lactate Assay Kit

55R-1450 Fitzgerald 100 assays 844 EUR

Lactate Assay Kit

55R-1469 Fitzgerald 100 assays 844 EUR

Human Lactate Dehydrogenase B (LDHB) ELISA Kit

SEB698Hu-10x96wellstestplate Cloud-Clone 10x96-wells test plate 4502.43 EUR

Human Lactate Dehydrogenase B (LDHB) ELISA Kit

SEB698Hu-1x48wellstestplate Cloud-Clone 1x48-wells test plate 458.44 EUR

Human Lactate Dehydrogenase B (LDHB) ELISA Kit

SEB698Hu-1x96wellstestplate Cloud-Clone 1x96-wells test plate 612.05 EUR

Human Lactate Dehydrogenase B (LDHB) ELISA Kit

SEB698Hu-5x96wellstestplate Cloud-Clone 5x96-wells test plate 2454.23 EUR

Human Lactate Dehydrogenase B (LDHB) ELISA Kit

4-SEB698Hu Cloud-Clone
  • 4553.00 EUR
  • 2405.00 EUR
  • 613.00 EUR
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells

Human Lactate Dehydrogenase A (LDHA) ELISA Kit

SEB370Hu-10x96wellstestplate Cloud-Clone 10x96-wells test plate 4502.43 EUR

Human Lactate Dehydrogenase A (LDHA) ELISA Kit

SEB370Hu-1x48wellstestplate Cloud-Clone 1x48-wells test plate 458.44 EUR

Human Lactate Dehydrogenase A (LDHA) ELISA Kit

SEB370Hu-1x96wellstestplate Cloud-Clone 1x96-wells test plate 612.05 EUR

Human Lactate Dehydrogenase A (LDHA) ELISA Kit

SEB370Hu-5x96wellstestplate Cloud-Clone 5x96-wells test plate 2454.23 EUR

Human Lactate Dehydrogenase A (LDHA) ELISA Kit

4-SEB370Hu Cloud-Clone
  • 4553.00 EUR
  • 2405.00 EUR
  • 613.00 EUR
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells

Human Lactate Dehydrogenase D (LDHD) ELISA Kit

SEE130Hu-10x96wellstestplate Cloud-Clone 10x96-wells test plate 4731.5 EUR

Human Lactate Dehydrogenase D (LDHD) ELISA Kit

SEE130Hu-1x48wellstestplate Cloud-Clone 1x48-wells test plate 477.3 EUR

Human Lactate Dehydrogenase D (LDHD) ELISA Kit

SEE130Hu-1x96wellstestplate Cloud-Clone 1x96-wells test plate 639 EUR

Human Lactate Dehydrogenase D (LDHD) ELISA Kit

SEE130Hu-5x96wellstestplate Cloud-Clone 5x96-wells test plate 2575.5 EUR

Human Lactate Dehydrogenase D (LDHD) ELISA Kit

4-SEE130Hu Cloud-Clone
  • 4782.00 EUR
  • 2526.00 EUR
  • 640.00 EUR
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells

Human Lactate Dehydrogenase C (LDHC) ELISA Kit

SEE131Hu-10x96wellstestplate Cloud-Clone 10x96-wells test plate 4731.5 EUR

Human Lactate Dehydrogenase C (LDHC) ELISA Kit

SEE131Hu-1x48wellstestplate Cloud-Clone 1x48-wells test plate 477.3 EUR

Human Lactate Dehydrogenase C (LDHC) ELISA Kit

SEE131Hu-1x96wellstestplate Cloud-Clone 1x96-wells test plate 639 EUR

Human Lactate Dehydrogenase C (LDHC) ELISA Kit

SEE131Hu-5x96wellstestplate Cloud-Clone 5x96-wells test plate 2575.5 EUR

Human Lactate Dehydrogenase C (LDHC) ELISA Kit

4-SEE131Hu Cloud-Clone
  • 4782.00 EUR
  • 2526.00 EUR
  • 640.00 EUR
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells

Human lactate dehydrogenase A (LDHA) ELISA kit

CSB-E17850h-24T Cusabio 1 plate of 24 wells 165 EUR

Human lactate dehydrogenase A (LDHA) ELISA kit

1-CSB-E17850h Cusabio
  • 900.00 EUR
  • 5476.00 EUR
  • 2900.00 EUR
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each

Human Lactate Dehydrogenase A (LDHA) ELISA Kit

RD-LDHA-Hu-48Tests Reddot Biotech 48 Tests 500 EUR

Human Lactate Dehydrogenase A (LDHA) ELISA Kit

RD-LDHA-Hu-96Tests Reddot Biotech 96 Tests 692 EUR

Human Lactate Dehydrogenase B (LDHB) ELISA Kit

RD-LDHB-Hu-48Tests Reddot Biotech 48 Tests 500 EUR

Human Lactate Dehydrogenase B (LDHB) ELISA Kit

RD-LDHB-Hu-96Tests Reddot Biotech 96 Tests 692 EUR

Human Lactate Dehydrogenase C (LDHC) ELISA Kit

RD-LDHC-Hu-48Tests Reddot Biotech 48 Tests 521 EUR

Human Lactate Dehydrogenase C (LDHC) ELISA Kit

RD-LDHC-Hu-96Tests Reddot Biotech 96 Tests 723 EUR

Human Lactate Dehydrogenase D (LDHD) ELISA Kit

RD-LDHD-Hu-48Tests Reddot Biotech 48 Tests 521 EUR

Human Lactate Dehydrogenase D (LDHD) ELISA Kit

RD-LDHD-Hu-96Tests Reddot Biotech 96 Tests 723 EUR

ELISA kit for Human LDH (Lactate Dehydrogenase)

ELK2236 ELK Biotech 1 plate of 96 wells 432 EUR

Human Lactate Dehydrogenase A (LDHA) ELISA Kit

DLR-LDHA-Hu-48T DL Develop 48T 498 EUR

Human Lactate Dehydrogenase A (LDHA) ELISA Kit

DLR-LDHA-Hu-96T DL Develop 96T 647 EUR

Human Lactate Dehydrogenase B (LDHB) ELISA Kit

DLR-LDHB-Hu-48T DL Develop 48T 498 EUR

Human Lactate Dehydrogenase B (LDHB) ELISA Kit

DLR-LDHB-Hu-96T DL Develop 96T 647 EUR

Human Lactate Dehydrogenase C (LDHC) ELISA Kit

DLR-LDHC-Hu-48T DL Develop 48T 517 EUR

Human Lactate Dehydrogenase C (LDHC) ELISA Kit

DLR-LDHC-Hu-96T DL Develop 96T 673 EUR

Human Lactate Dehydrogenase D (LDHD) ELISA Kit

DLR-LDHD-Hu-48T DL Develop 48T 517 EUR

Human Lactate Dehydrogenase D (LDHD) ELISA Kit

DLR-LDHD-Hu-96T DL Develop 96T 673 EUR

Human Lactate Dehydrogenase A (LDHA) ELISA Kit

abx573441-96tests Abbexa 96 tests 668 EUR

Human Lactate Dehydrogenase C (LDHC) ELISA Kit

abx573952-96tests Abbexa 96 tests 668 EUR

Human Lactate Dehydrogenase B (LDHB) ELISA Kit

abx575224-96tests Abbexa 96 tests 668 EUR

Human Lactate Dehydrogenase D (LDHD) ELISA Kit

abx053234-96tests Abbexa 96 tests 754 EUR

Human Lactate Dehydrogenase A (LDHA) ELISA Kit

20-abx152179 Abbexa
  • 7112.00 EUR
  • 3792.00 EUR
  • 879.00 EUR
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Lactate Dehydrogenase B (LDHB) ELISA Kit

20-abx152180 Abbexa
  • 7112.00 EUR
  • 3792.00 EUR
  • 879.00 EUR
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Lactate Dehydrogenase C (LDHC) ELISA Kit

20-abx152181 Abbexa
  • 7378.00 EUR
  • 3933.00 EUR
  • 911.00 EUR
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Lactate Dehydrogenase D (LDHD) ELISA Kit

20-abx152182 Abbexa
  • 7378.00 EUR
  • 3933.00 EUR
  • 911.00 EUR
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Lactate Dehydrogenase A (LDHA) ELISA Kit

abx251492-96tests Abbexa 96 tests 668 EUR

Human Lactate Dehydrogenase C (LDHC) ELISA Kit

abx251493-96tests Abbexa 96 tests 668 EUR
  • The study included 24 asthmatics, 36 patients with COPD, and 12 controls. TSLP concentration was measured with the use of R&D and EIAab commercial ELISA kits.
  • The results obtained with the EIAab kit were 3 to even 45-fold higher than those measured with the R&D kit. Significant differences between the investigated groups were found only for the TSLP concentration in induced sputum. When the R&D kit was used, the highest TSLP levels in induced sputum were found in asthmatics, while the EIAab kit showed the highest TSLP levels in controls.
  • The distribution of results in the Bland-Altman plot was typical for a proportional constant error. TSP concentration in induced sputum might be a more reliable asthma biomarker than serum TSLP. We conclude that TSLP level is highly dependent on the ELISA kit used for the measurement.
  • Thus, judgement on TSLP results obtained with different assays might be confusing and lead to wrong conclusions.

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